TruAge vs GlycanAge vs DoNotAge: Comparing the Best Biological Age Tests

You’ve probably heard it by now: your biological age matters more than the number of candles on your last birthday cake. Knowing how old your body really is isn’t just interesting: it’s powerful data you can use to improve your health. Three tests lead the space in 2025: TruAge vs GlycanAge vs DoNotAge. In this guide, we compare them head-to-head so you can choose the best biological age test for your goals.

What do TruAge, GlycanAge, and DoNotAge age tests actually measure?

TruAge uses blood-based DNA methylation to assess aging speed and organ health. GlycanAge analyzes immune and inflammation aging through IgG glycans. DoNotAge offers a saliva test with broader wellness markers like memory and vision.

TruAge (TruDiagnostic)

TruAge measures biological age using DNA methylation. Developed alongside top research institutions like Harvard, Yale, Duke, and Columbia, it leverages industry-leading algorithms: OMICmAge, DunedinPACE, and SYMPHONYAge.
These give insights not just into your biological age but also how fast you’re aging, organ-specific aging, and even telomere length.

• Sample type: Blood (finger-prick)
• Turnaround: 3–4 weeks
• Accuracy: Validated in multiple peer-reviewed studies; predictive accuracy around 98–99%

GlycanAge

GlycanAge approaches biological age differently, it measures glycans attached to immunoglobulin G (IgG), reflecting your immune health and inflammation levels. Glycans reveal how lifestyle and inflammation impact your aging process, making it sensitive to lifestyle shifts.

• Sample type: Blood (finger-prick)
• Turnaround: 3–5 weeks
• Accuracy: Validated by decades of glycoscience research, widely used in clinical studies (Harvard)

DoNotAge

DoNotAge relies on DNA methylation through saliva samples and adds metrics like inflammation, memory, hearing, and eye health. It’s designed as a broader wellness test rather than purely a biological age clock.

• Sample type: Saliva
• Turnaround: Up to 12 weeks
• Accuracy: Proprietary machine-learning algorithms; less academically validated compared to TruAge or GlycanAge, but provides a holistic wellness snapshot (NIH | DNA methylation)

Head-to-Head Comparison: TruAge vs GlycanAge vs DoNotAge

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TruAge, GlycanAge, and DoNotAge Discount Codes

Which biological age test is best for you?

Choose TruAge for detailed metrics, GlycanAge for immune tracking, or DoNotAge for a broad health overview.

• TruAge: If you’re a biohacker or longevity enthusiast looking for precise organ-level insights and comprehensive aging metrics, TruAge is your gold standard (Harvard on biological clocks).
• GlycanAge: Ideal if you’re particularly interested in inflammation, immune system aging, or hormonal impacts like menopause or stress.
• DoNotAge: Good if you want a broader wellness snapshot beyond just biological age, measuring additional lifestyle markers like memory and sensory aging.

What can you do with your biological age results?

Use them to guide targeted lifestyle changes, whether it’s cardio for heart health, anti-inflammatory diets, or memory-boosting habits, based on what your test reveals.

One of the biggest mistakes people make is getting their biological age score and then doing nothing with it. Here’s exactly how you can apply these insights into everyday life:

TruAge: Precision Lifestyle Changes

TruAge gives you a detailed breakdown of how each organ system is aging. Let’s say your report flags your cardiovascular system as aging faster than the rest of your body. You’d want to prioritize lifestyle adjustments like introducing more cardio exercise, heart-healthy foods, and stress management techniques. Harvard researchers confirm that targeted lifestyle interventions can measurably reverse signs of biological aging (Harvard Study on Aging Reversal, 2023).

Similarly, if your immune system or inflammation scores are concerning, that could mean you might benefit from anti-inflammatory supplements (like omega-3 or curcumin), more consistent sleep schedules, or even fasting protocols proven to reduce inflammation markers. The goal isn’t perfection: it’s actionable, incremental improvements tailored exactly to what your biology says you need.

GlycanAge: Adjust Your Habits, Track Progress

With GlycanAge, your insights focus specifically on inflammation and immune health. Imagine your GlycanAge results reveal you’re biologically older due to chronic stress. Science consistently links stress to accelerated immune aging (NIH Study on Stress and Immune Aging, 2022). A practical response would be incorporating a structured stress-relief routine like meditation, yoga, or even daily nature walks. Retesting 6 months later helps you quantify exactly how these new habits impacted your inflammatory biomarkers and immune health.

GlycanAge’s clear-cut sensitivity to lifestyle shifts means it’s especially powerful for anyone actively experimenting with dietary changes or stress-management strategies. Each follow-up test tells you exactly how your choices are moving the needle, giving you constant motivation to stay consistent.

DoNotAge: Wellness Snapshot for Broad Changes

DoNotAge takes a broader approach, offering additional insights into memory, vision, hearing, and overall inflammation. Say your results indicate your memory age is significantly older than your chronological age. This doesn’t mean you’re losing your mind, but it does suggest a proactive approach. Studies show lifestyle factors significantly influence cognitive aging (NIH on Lifestyle and Cognitive Aging, 2024).

You could start by incorporating brain-boosting foods like blueberries and walnuts, or simple daily brain-training exercises. Additionally, your inflammation scores might prompt you to adopt dietary strategies, such as a Mediterranean diet, known to reduce systemic inflammation and support cognitive health. Re-testing annually helps you track if your memory and inflammation metrics improve, making this test ideal for those wanting regular, broad-spectrum health checks rather than laser-focused precision.

Why Choosing the Right Epigenetic Test Matters

Consider two scenarios that show clearly how picking the right test can drastically change your outcome:

Scenario A: John (age 45, fitness-focused)
John chose TruAge because he’s been optimizing fitness and health. His TruAge test showed his biological age as slightly younger, but his cardiovascular age lagged behind due to genetic predispositions. This data prompted John to fine-tune his fitness regime, incorporating more cardio-intensive workouts and nutritional tweaks focused on heart health. After a year, his follow-up showed marked improvements in his cardiovascular scores, proving the targeted approach genuinely worked.

Scenario B: Sarah (age 53, high-stress job)
Sarah opted for GlycanAge, suspecting stress was prematurely aging her. Sure enough, her biological age came back significantly older, flagged by elevated inflammatory markers. Sarah used these results as motivation to adjust her lifestyle radically. She started daily mindfulness sessions and introduced anti-inflammatory foods like turmeric and olive oil. Retesting nine months later, her GlycanAge scores dropped by 5 years biologically, a tangible result directly connected to her actionable lifestyle changes.

These real-life cases underline why your choice between TruAge, GlycanAge, and DoNotAge matters. Your biological age isn’t just a number, it’s a roadmap for real, meaningful change.

Written by the editorial team at Simply Anti-Aging.
Reviewed for accuracy and updated in July 2025.